Diagnostic Cytogenetics

Rolf-Dieter Wegner

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English

Springer-Verlag Berlin and Heidelberg GmbH & Co. K
15 April 2014

Cytology; Scientific equipment, experiments & techniques; Genetics (non-medical); Cellular biology (cytology)

Series: Springer Lab Manuals

In addition to the traditional cytogenetics still used as the basic methodology for everyday clinical diagnosis, new molecular cytogenetic techniques provide a useful basis for routine diagnosis. Flourescence in situ hybridization (FISH) has become a standard technique, and comparative genomic hybridization (CGH), spectral karyotyping (SKY), and multi-color FISH have shown their potential for diagnostic purposes. Following a section on tissue culture, chromosome staining and basic information about karyotyping, nomenclature and quality standards, protocols of relevance for comprehensive cytogenetic diagnostics are presented.

Edited by: Rolf-Dieter Wegner
Imprint: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Country of Publication: Germany
Edition: 1999 ed.
Dimensions: Height: 254mm, Width: 203mm, Spine: 24mm
Weight: 1.024kg
ISBN: 9783642478130
ISBN 10: 3642478131
Series: Springer Lab Manuals
Pages: 460
Publication Date: 15 April 2014
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active

I Classical Cytogenetics.- 1 Tissue Culture.- 2 Chromosome Staining.- 3 Karyotyping and Data Interpretation.- 4 Documentation.- II Postnatal Diagnosis.- 5 Peripheral Blood.- 6 Establishment of Permanent Growing Lymphoblastoid Cell Lines.- 7 Solid Tissues.- 8 Cells from Urine Sample.- 9 Classical and Molecular Cytogenetics of Tumor Cells.- 10 Cytogenetics of Meiotic Cells.- III Prenatal Diagnosis.- 11 Prenatal Diagnosis — An Introduction.- 12 Amniotic Fluid Cell Analysis.- 13 Chorionic Villi Sampling.- IV Special Applications.- 14 Diagnosis of Chromosomal Instability Syndromes.- 15 Flow Cytometric Testing for Syndromes with Chromosomal Instability, Aplastic Anemia and Related Hematological Disorders.- 16 Cell Fusion.- 17 Origin of Trisomies.- V Molecular Cytogenetics.- 18 Fluorescence in Situ Hybridization.- 19 Fluorescence in Situ Hybridization (FISH) Analysis in Human Sperm Cells.- 20 Microdissection and Reverse Chromosome Painting.- 21 Comparative genomic hybridisation (CGH).- VI Techniques in Development.- 22 Fetal Cells in Maternal Blood.- 23 Spectral Karyotyping in Clinical and Tumor Cytogenetics.- 24 Chromosome Analysis by Multiplex-FISH (M-FISH).

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Diagnostic Cytogenetics

Rolf-Dieter Wegner

$260.95 $208.95

Paperback

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