Decoding the Antibody Repertoire: High Throughput Sequencing of Multiple Transcripts from Single B Cells...

— —

Brandon DeKosky

Decoding the Antibody Repertoire

High Throughput Sequencing of Multiple Transcripts from Single B Cells

Brandon DeKosky

$251.95 $201.58

Hardback

Not in-store but you can order this
How long will it take?

Availability Information

We source books from suppliers in Australia and overseas. For books we don't currently have in stock, the time it takes to get them from our suppliers can vary widely - from a few days to a few months - so we check each book with each supplier to determine the expected time it will take to be supplied to us.

We then advise you accordingly. If the time taken to get any book is too long for you, you can let us know and we will cancel or adjust your order, and refund as required.

To find out the anticipated arrival time for specific items prior to ordering, please contact us by phone or email:

Phone +61 2 9264 3111, or 1800 4 BOOKS (1800 4 26657) if outside Sydney:
option 1 Abbey's Bookshop (Crime, History, Science, Kids & more) • info@abbeys.com.au
option 2 Language Book Centre (ESL & Foreign Languages) • language@abbeys.com.au
option 3 Galaxy Bookshop (Sci-fi, Fantasy, Romance, Graphic Novels) • sf@galaxybooks.com.au

QTY:

English

Springer International Publishing AG
08 June 2017

Genetics (non-medical); Genetic engineering

Series: Springer Theses

This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening an entirely new window for antibody discovery and the investigation of adaptive immune responses to vaccines and diseases.

Previous methods for high-throughput immune repertoire sequencing have been unable to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. The author directly addresses these limitations by designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells.

The techniques developed in this work have enabled comprehensive interrogation of human B-cell repertoires and have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.

By: Brandon DeKosky
Imprint: Springer International Publishing AG
Country of Publication: Switzerland
Edition: 1st ed. 2017
Dimensions: Height: 235mm, Width: 155mm,
Weight: 454g
ISBN: 9783319585178
ISBN 10: 3319585177
Series: Springer Theses
Pages: 87
Publication Date: 08 June 2017
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

Brandon DeKosky is an assistant professor at the Departments of Pharmaceutical Chemistry, Chemical & Petroleum Engineering, and the Kansas Vaccine Institute at the University of Kansas. Dr. DeKosky attained his Ph.D. in the lab of George Georgiou at the University of Texas at Austin where he developed new technologies for sequencing complete antibody variable regions from single B cells. Dr. DeKosky performed postdoctoral studies in the lab of John Mascola at the NIAID Vaccine Research Center, applying high-throughput technologies to accelerate public health vaccine development. His lab at KU seeks to leverage high-throughput platforms to understand the features of adaptive immune protection and develop novel strategies to combat human diseases.