CRISPR-Cas Systems for RNA and Genome Editing, Volume 711 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites, Genome oligopaint via local denaturation fluorescence in situ hybridization, Biochemical reconstitution of a type I-B CRISPR-associated transposon, Visualizing the conformational landscape of
CRISPR-Cas9 through kinetics-informed structural studies, and much more.
Additional sections cover Analysis of metal-dependent DNA nicking activities by Cas endonucleases, Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems, Prime editing in bacteria with BacPE, Preparation of high-purity CRISPR-based DNA base editor RNPs, Bacterial directed evolution of CRISPR base editors, Cloning and validating systems for high throughput molecular recording, Using CRISPR-Cas13 for viral nucleic acid detection, and more.
Volume editor:
Ailong Ke,
Weixin Tang
Imprint: Academic Press Inc
Country of Publication: United States
Dimensions:
Height: 229mm,
Width: 152mm,
Weight: 1.070kg
ISBN: 9780443317521
ISBN 10: 0443317526
Series: Methods in Enzymology
Pages: 576
Publication Date: 02 June 2025
Audience:
Professional and scholarly
,
Undergraduate
Format: Hardback
Publisher's Status: Active
1. Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites TJ Ha 2. Genome oligopaint via local denaturation fluorescence in situ hybridization TJ Ha 3. TBD Osamu Nureki 4. Biochemical reconstitution of a type I-B CRISPR-associated transposon Leifu Chang 5. TBD Yibei Xiao 6. Visualising the conformational landscape of of CRISPR-Cas9 through kinetics-informed structural studies David W. Taylor 7. Analysis of metal-dependent DNA nicking activities by Cas endonucleases Dipali G. Sashital 8. Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems. Ryan Jackson 9. HYER Junjie (Gogo) liu 10. focused on the purification and characterization of gRAMP, as well as its application in GFP knockdown in E. coli. Chunyi Hu 11. TBD Peter Fineran 12. Cas12a2 ? Chase Beisel 13. Prime editing in bacteria with BacPE Quanjiang Ji 14. TBD Jia Chen 15. Preparation of high-purity CRISPR-based DNA base editor RNPs Audrone Lapinaite 16. Bacterial directed evolution of CRISPR base editors Shannon Miller 17. Cloning and validating systems for high throughput molecular recording Michelle Chan 18. TBD Samagya Banskota 19. Using CRISPR-Cas13 for viral nucleic acid detection Cameron Myhrvold 20. CRISPR-based epigenetic editing for programmable gene silencing in mammalian cells James Nuñez 21. Genome editing with programmable base editors in human cells” – explaining how to pick out and validate BE:gRNA combinations for a given target, with tips and tricks for maximizing editing efficiencies Alexis Komor 22. High-throughput evaluation of genetic variants using base/prime editing sensor libraries Francisco J. Sánchez Rivera 23. TBD Jia Chen
