Comparative Biology of Aging

Norman S. Wolf

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English

Springer
12 November 2014

Maturation & ageing; Cellular biology (cytology)

Summary
Details

determined by an inability to move in response to touch. C. elegans develop through four larval stages following hatching and prior to adulthood. Adult C. elegans are reproductive for about the rst week of adulthood followed by approximately two weeks of post-reproductive adulthood prior to death. Life span is most commonly measured in the laboratory by maintaining the worms on the surface of a nutrie- agar medium (Nematode Growth Medium, NGM) with E. coli OP50 as the bacterial food source (REF). Alternative culture conditions have been described in liquid media; however, these are not widely used for longevity studies. Longevity of the commonly used wild type C. elegans hermaphrodite (N2) varies ? from 16 to 23 days under standard laboratory conditions (20 C, NGM agar, E. coli OP50 food source). Life span can be increased by maintaining animals at lower ambient temperatures and shortened by raising the ambient temperature. Use of a killed bacterial food source, rather than live E. coli, increases lifespan by 2–4 days, and growth of adult animals in the absence of bacteria (axenic growth or bac- rial deprivation) increases median life span to 32–38 days [3, 23, 24]. Under both standard laboratory conditions and bacterial deprivation conditions, wild-derived C. elegans hermaphrodites exhibit longevity comparable to N2 animals [25].

Edited by: Norman S. Wolf
Imprint: Springer
Edition: 2010 ed.
Dimensions: Height: 235mm, Width: 155mm, Spine: 21mm
Weight: 617g
ISBN: 9789400790834
ISBN 10: 940079083X
Pages: 391
Publication Date: 12 November 2014
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active