Coherent Raman Scattering Microscopy

Ji-Xin Cheng, Xiaoliang Sunney Xie

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English

CRC Press Inc
29 October 2012

Biomedical engineering; Microscopy; Physics; Spectrum analysis, spectrochemistry, mass spectrometry; Technology, Engineering & Agriculture

Series: Series in Cellular and Clinical Imaging

The First Book on CRS Microscopy

Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Scattering Microscopy explains how CRS can be used to obtain a point-by-point chemical map of live cells and tissues.

In color throughout, the book starts by establishing the foundation of CRS microscopy. It discusses the principles of nonlinear optical spectroscopy, particularly coherent Raman spectroscopy, and presents the theories of contrast mechanisms pertinent to CRS microscopy. The text then provides important technical aspects of CRS microscopy, including microscope construction, detection schemes, and data analyses. It concludes with a survey of applications that demonstrate how CRS microscopy has become a valuable tool in biomedicine.

Due to its label-free, noninvasive examinations of living cells and organisms, CRS microscopy has opened up exciting prospects in biology and medicine—from the mapping of 3D distributions of small drug molecules to identifying tumors in tissues. An in-depth exploration of the theories, technology, and applications, this book shows how CRS microscopy has impacted human health and will deepen our understanding of life processes in the future.

Edited by: Ji-Xin Cheng, Xiaoliang Sunney Xie
Imprint: CRC Press Inc
Country of Publication: United States
Dimensions: Height: 254mm, Width: 178mm, Spine: 28mm
Weight: 1.451kg
ISBN: 9781439867655
ISBN 10: 1439867658
Series: Series in Cellular and Clinical Imaging
Pages: 610
Publication Date: 29 October 2012
Audience: College/higher education , General/trade , Primary , ELT Advanced
Format: Hardback
Publisher's Status: Active

Theory: Theory of Coherent Raman Scattering. Coherent Raman Scattering under Tightly Focused Conditions. Platforms: Construction of a Coherent Raman Microscope. Stimulated Raman Scattering Microscopy. Femtosecond versus Picosecond Pulses for Coherent Raman Microscopy. Wide-Field CARS Microscopy. Vibrational Spectromicroscopy by Coupling Coherent Raman Imaging with Spontaneous Raman Spectral Analysis. Coherent Control in CARS. Fourier Transform CARS Microscopy. CRS with Alternative Beam Profiles. Vibrational Phase Microscopy. Multiplex CARS Microscopy. Interferometric Multiplex CARS. Photonic Crystal Fiber-Based Broadband CARS Microscopy. Multiplex Stimulated Raman Scattering Microscopy. Applications: Imaging Myelin Sheath Ex Vivo and In Vivo by CARS Microscopy. Imaging Lipid Metabolism in Caenorhabditis elegans and Other Model Organisms. Lipid-Droplet Biology and Obesity-Related Health Risks. White Matter Injury: Cellular-Level Myelin Damage Quantification in Live Animals. CARS Microscopy Study of Liquid Crystals. Live Cell Imaging by Multiplex CARS Microspectroscopy. Coherent Raman Scattering Imaging of Drug Delivery Systems. Applications of Stimulated Raman Scattering Microscopy. Applications of Coherent Anti-Stokes Raman Spectroscopy Imaging to Cardiovascular Diseases. Applications of CARS Microscopy to Tissue Engineering. Dietary Fat Absorption Visualized by CARS Microscopy. Index.

Ji-Xin Cheng is a professor of biomedical engineering at Purdue University. He earned a PhD from the University of Science and Technology of China and completed postdoctoral research at the Hong Kong University of Science and Technology and Harvard University. He is a pioneer in the development and biomedical application of molecular vibration-based imaging tools. Xiaoliang Sunney Xie is a Mallinckrodt Professor of Chemistry at Harvard University. He earned a PhD from the University of California-San Diego and completed postdoctoral research at the University of Chicago. He is well-known for his innovations in nonlinear Raman microscopy and his pioneering work in single-molecule biophysical chemistry, including enzyme dynamics and live cell gene expression.

Reviews for Coherent Raman Scattering Microscopy

"""This book makes a seminal contribution to the field of biomedical imaging, and provides the reader with an excellent introduction and in-depth descriptions of the latest and greatest implementations of coherent Raman imaging technology—enough to jump start a new program or infuse new life into an existing one. The clear descriptions of a wide range of applications show how the methods are being used at present and indicate many different and varied future paths. This book is an excellent addition to the bookshelves of biomedical imaging practitioners and researchers in related fields, including surface analysis, process control, forensics, and others."" —David S. Moore, Los Alamos National Laboratory, Analytical and Bioanalytical Chemistry, 2013 ""Interested readers can find an excellent mix of [optical platforms and applications given a solid theoretical approach] in this book, which is the first one dealing in a comprehensive and exhaustive way with CRS microscopy and related techniques. No one involved in optical microscopy and spectroscopy should miss this text, which is able to offer new exciting scenarios and, for sure, at least one new idea after reading."" —Alberto Diaspro, Italian Institute of Technology (IIT), Journal of Biomedical Optics, 2014"