This detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through maintaining the native physiological context through the investigation of living, functional cells. In addition, the chapters reflect the dramatic improvement in the length scale of precision by utilizing single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA as well as using methods that can controllably manipulate single DNA molecules, and the use of advanced computational methods and mathematical analysis is also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.
Edited by:
Mark C. Leake Imprint: Springer-Verlag New York Inc. Country of Publication: United States Edition: 2nd ed. 2022 Volume: 2476 Dimensions:
Height: 254mm,
Width: 178mm,
Weight: 845g ISBN:9781071622209 ISBN 10: 107162220X Series:Methods in Molecular Biology Pages: 330 Publication Date:31 May 2022 Audience:
Professional and scholarly
,
Undergraduate
Format:Hardback Publisher's Status: Active