This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells.
1. Correlative Light and Electron Microscopy Analysis of the Centrosome; A Step by Step Protocol. Dong Kong and Jadranka Loncarek 2.Generation of a Conditional Analog-Sensitive Kinase in Human Cells Using CRISPR/Cas9-Mediated Genome Engineering Tyler Moyer and Andrew J. Holland 3. Studying Centrosome Function Using Three-Dimensional Cell Cultures Teresa Arnandis and Susana A. Godinho 4. Analysis of Centrosomes in Human Cancer Stefan Duensing 5. In Vivo Examination of the Cortical Cytoskeleton in Multiciliated Cells Using Electron Tomography Daniel K. Clare, Maud Dumoux and Delphine Delacour 6.Tackling Centrosome Biology Through Gene Targeting in Chicken B Cells Pavithra L. Chavali and Fanni Gergely 7. Centriole Biogenesis and Function in Multi-Ciliated Cells Siwei Zhang and Brian J. Mitchell 8. Sub-Diffraction Resolution Microscopy Methods for Analyzing Centrosomes Organization Vito Mennella, Rachel Hanna and Moshe Kim 9. Probing Mammalian Centrosome Structure Using Bioid Proximity-Dependent Biotinylation Elif Nur Firat-Karalar and Tim Stearns 10. Purification of Centrosomes from Mammalian Cell Lines Delphine Gogendeau, Paul Guichard and Anne-Marie Tassin 11. Isolation, Cryo-Tomography and Three-Dimensional Reconstruction of Centrioles Paul Guichard, Virginie Hamel, Aitana Neves and Pierre Gönczy 12. New Insights into Centrosome Imaging in Drosophila and Mouse Neuroepithelial Tissues Maria A. Rujano, Renata Basto and Véronique Marthiens 13. Microinjection Techniques for Studying Centrosome Function in Drosophila Melanogaster Syncytial Embryos Paul T. Conduit, Daniel Hayward and James G. Wakefield 14. A Yeast Two-Hybrid Approach For Probing Protein-Protein Interactions at the Centrosome Brian J. Galletta1 and Nasser M. Rusan 15. Genome-Wide RNAi Screens in S2 Cells To Identify Centrosome Components Jeroen Dobbelaere 16. Time- Lapse Recording of Centrosomes and Other Organelles in Drosophila Neuroblasts Judit Pampalona, Jens Januschke, Paula Sampaio, and Cayetano Gonzalez 17. Centrosomes and Spindles in Ascidian Embryos and Eggs Alex McDougall, Janet Chenevert, Gerard Pruliere, Vlad Costache, Celine Hebras, Gregory Salez and Remi Dumollard 18. Ultrastructural Analysis of C. elegans Cilia Daniel Serwas and Alexander Dammermann 19. Method: In Vitro Analysis of Pericentriolar Material Assembly Jeffrey B. Woodruff and Anthony A. Hyman 20. Monitoring SPB Biogenesis in Fission Yeast with High Resolution and Quantitative Fluorescent Microscopy Imène B. Bouhlel, Kathleen Scheffler, Phong T. Tran and Anne Paoletti
Reviews for Centrosome and Centriole
Praise for the Series: The series is invaluable for workers at all levels of cell biology. --Nature
