Cell-Free Gene Expression

Methods and Protocols

Ashty S. Karim, Michael C. Jewett

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English

Springer-Verlag New York Inc.
07 January 2023

Scientific equipment, experiments & techniques; Genetics (non-medical); Biochemistry; Biotechnology

Series: Methods in Molecular Biology

Summary
Details

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications.

Edited by: Ashty S. Karim, Michael C. Jewett
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 1st ed. 2022
Volume: 2433
Dimensions: Height: 254mm, Width: 178mm,
Weight: 861g
ISBN: 9781071620007
ISBN 10: 1071620002
Series: Methods in Molecular Biology
Pages: 435
Publication Date: 07 January 2023
Audience: College/higher education , Further / Higher Education
Format: Paperback
Publisher's Status: Active