Biological Aging

Methods and Protocols

Trygve O. Tollefsbol

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English

Humana Press Inc.
27 November 2014

Maturation & ageing; Developmental biology; Cellular biology (cytology)

Series: Methods in Molecular Biology

Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. The editors have carefully selected only those topics that are considered mainstays of the field or are showing promise in revolutionizing this relatively new science. The three main areas of focus in this cutting-edge compendium of biological aging research are: methods that are basic to understanding the fundamental mechanisms of cellular aging; techniques used to intervene in the aging process; and approaches to analyzing the many molecular processes of biological aging. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions in the exciting and expanding field of biological aging.

Edited by: Trygve O. Tollefsbol
Imprint: Humana Press Inc.
Country of Publication: United States
Edition: 2007 ed.
Volume: 371
Dimensions: Height: 235mm, Width: 155mm, Spine: 22mm
Weight: 658g
ISBN: 9781627039062
ISBN 10: 1627039066
Series: Methods in Molecular Biology
Pages: 414
Publication Date: 27 November 2014
Audience: College/higher education , Further / Higher Education
Format: Paperback
Publisher's Status: Active

Techniques for Analysis of Biological Aging.- Aging Cell Culture.- Methods to Detect Biomarkers of Cellular Senescence.- Methods for Cell Sorting of Young and Senescent Cells.- Telomere Length Analysis.- A Method to Detect DNA Methyltransferase I Gene Transcription In Vitro in Aging Systems.- A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro.- The Chronological Life Span of Saccharomyces cerevisiae.- Caloric Restriction and Life Span Determination of Yeast Cells.- Methods for Nutrigenomics and Longevity Studies in Drosophila.- Caloric Restriction Mimetics.- Extension of Cell Life Span Using Exogenous Telomerase.- Inducing Cellular Senescence Using Defined Genetic Elements.- Methods of Cellular Senescence Induction Using Oxidative Stress.- Nuclear Transfer Methods to Study Aging.- Use of Ribozymes in Cellular Aging Research.- Methods of Evaluating the Effect of Pharmacological Drugs On Aging and Life Span in Mice.- Identifying Genes that Extend Life Span Using a High-Throughput Screening System.- Application of DNA Microarray Technology to Gerontological Studies.- Detection and Analysis of Somatic Mutations at a lacZ Reporter Locus in Higher Organisms.- Subtractive Hybridization Techniques to Study Cellular Senescence.- Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity.- Quantitative Trait Locus (QTL) Mapping in Aging Systems.- 2D Gel Proteomics.- Metabolomics in the Study of Aging and Caloric Restriction.

Reviews for Biological Aging: Methods and Protocols

From the reviews: For research gerontologists, this book is a boon. -Doody's Book Review, Weighted Numerical Score:90 - 4 Stars This can be a useful book for biogerontologists. ... Comprised of 25 generally well-written articles ... this book covers various methods and protocols for studying cellular aging of human cells, yeast cells, and mice. ... a welcome source of useful information for researchers intending to find their way into descriptive, analytical and interventive research in biogerontology. (Suresh Rattan, Biogerontology, Issue 9, 2008)