The book provides a comprehensive guide that covers the fundamental principles and methodologies of essential bioanalytical techniques.
Bioanalytical Techniques: Principles and Applications is a comprehensive and authoritative book that explores the principles, methodologies, and applications of bioanalytical techniques in the field of life sciences. The book covers a wide range of analytical techniques used for the characterization, quantification, and analysis of biological samples, including proteins, nucleic acids, metabolites, and biomarkers. Using a multidisciplinary approach by integrating concepts from biochemistry, molecular biology, analytical chemistry, and biotechnology, this book provides a solid foundation in the fundamental principles underlying various bioanalytical techniques, such as spectroscopy, chromatography, electrophoresis, immunoassays, mass spectrometry, and biosensors. Each technique is explained in detail, including its working principles, instrumentation, data analysis, and practical considerations. The book incorporates case studies, examples, and practical tips to illustrate how these techniques are used to solve biological problems and address research questions. It also discusses emerging trends and technologies in bioanalytical techniques, such as microfluidics, nanotechnology, and omics approaches.
Readers will find the book:
Offers comprehensive coverage of bioanalytical techniques, encompassing a wide range of methodologies, instruments, and applications through real-world case studies; Adopts a multidisciplinary approach, integrating concepts from biochemistry, molecular biology, analytical chemistry, and biotechnology; Explores emerging trends and technologies in bioanalytical techniques, such as microfluidics, nanotechnology, omics approaches, and bioinformatics; Includes practical guidance, troubleshooting tips, and common challenges in bioanalysis, equipping readers with valuable insights and strategies for successful experimentation and data interpretation; Features contributions from renowned experts and leaders in the field, ensuring the content is authoritative, up-to-date, and reflects the latest advancements in bioanalytical techniques.
Audience
Biochemists, biologists, chemists, and medical and pharmaceutical professionals interested in biomolecules, enzymology, and biochemical pathways.
Preface xxix 1 Point-of-Care Diagnostics for Rapid Disease Detection 1 Nabil H. Bhuiyan and M. Khalid Hossain 2 Principles of Molecular Biology for Bioanalysis 39 Syed Ali Raza Naqvi, Syeda Marab Saleem, Tania Jabbar, Atta Ul Haq, Muhammad Asif , Sadaf Ul Hassan, Muhammad Rehan Hasan Shah Gilani, Naseem Abbas and Ali Abbas 3 UV–Visible Spectroscopy for Quantitative Analysis of Biomolecules 79 Syeda Marab Saleem, Syed Ali Raza Naqvi, Muhammad Ramzan Saeed Ashraf Janjua, Tauqir A. Sherazi, Muhammad Saeed, Sadaf Ul Hassan, Muhammad Imran, Muhammad Asif, Mamoon Ur Rasheed and Tania Jabbar 4 Emerging Trends and Future Perspectives in Bioanalysis 119 Jayapal Reddy Gangadi, Kalpana Swain and Satyanarayan Pattnaik 5 Introduction to Biosensors for Point‐of-Care Testing: IoT-Based Vitamin D3 Biosensor 137 Asif Hussain Shaik and Chinna Babu Jyothi 6 Fluorescence Spectroscopy for Protein–Protein Interactions 149 Masoud Shayegan and Hossein Ahmadzadeh 7 Flow Cytometry for Cell Analysis and Sorting 173 Maryam Davardoostmanesh and Hossein Ahmadzadeh 8 Application in Drug Discovery and Development for Lead Identification 189 Sareena Ehsan, Rida Mumtaz, Abdul Rehman, Maryam, Habibullah Nadeem, Ammara Riaz, Jallat Khan, Ebrahim Alinia-Ahandani and Zeliha Selamoglu 9 Immunoblotting Techniques for Protein Identification and Quantification and Its Application 203 Megha Sikder, Somen Debnath and Biswanath Bhunia 10 Gel Electrophoresis for DNA Fragment Analysis 221 Aneela Nawaz, Shahzar Khan, Momina Aamir, Sidra Zaheer, Sabeena Zafar, Nauman Ahmed Khalid and Samiullah Khan 11 Omics Approaches in Bioanalysis for Systems Biology Studies 239 Rana Hooshang, Hoda Nouri and Hamid Moghimi 12 Introduction to Mass Spectrometry for Metabolite Profiling 283 Jackie E. Wood and Brendon D. Gill 13 Infections, Symptoms, and Clinical Diagnostic Techniques for Dengue: A Case Study of a Neglected Tropical Disease 341 Shreeganesh Subraya Hegde and Badekai Ramachandra Bhat 14 Microfluidic-Based Bioassays for High-Throughput Analysis 355 Muthui Martin Mwaurah, J. Mathiyarasu and A.M. Vinu Mohan 15 Circular Dichroism Spectroscopy for Secondary Structure Analysis of Proteins 383 Masooma Siddiqui, Maroof Ali, Mehak Sagheer and Mohd Imran Ahamed 16 Optical Biosensors for Detection of Analytes in Biological Samples 427 Uzma, Masooma Siddiqui and Mehtab Parveen 17 Tandem Mass Spectrometry for Peptide Sequencing 461 Satyendra Mishra and Neha V. Rathod 18 Unraveling Complexity: Cutting-Edge Ion Chromatography Methods for the Analysis of Inorganic Ions in Biological Samples 489 Muhammad Hafiznur Yunus, Nor Ain Shahera Khairi, Azren Aida Asmawi, Fatin Nabilah Mohd Faudzi, Ahmad Farabi Mohamad Saman, Nor Azah Yusof and Siti Fatimah Nur Abdul Aziz 19 Sample Preparation and Handling for Reliable Results 551 Virender, Rohit, Ashwani Kumar, Rakesh Kumar Gupta and Brij Mohan 20 Data Analysis in Bioanalysis for Interpretation of Experimental Data 575 Jaymin Parikh, Keyur Bhatt, Krunal Modi, Jasbir Sangwan, Alexander A. Solovev and Brij Mohan 21 Protein Identification Using Matrix-Assisted Laser Desorption/ Ionization Mass Spectrometry 605 Vipul D. Prajapati and Princy Shrivastav 22 Applications of Electrothermal Atomic Absorption Spectrometry (ET-AAS) Toward Various Metal Analyses in Diverse Biological and Environmental Matrices 629 Aruna Jyothi Kora 23 Thin-Layer Chromatography for Natural Product Analysis 645 Mahreen Imam, Syed Ali Raza Naqvi, Majid Muneer, Atta Ul Haq, Sadaf Ul Hassan, Muhammad Rehan Hasan Shah Gilani, Naseem Abbas and Syeda Marab Saleem Index 667
Inamuddin, PhD, is an assistant professor at the Department of Applied Chemistry, Zakir Husain College of Engineering and Technology, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh, India. He has extensive research experience in multidisciplinary fields of analytical chemistry, materials chemistry, electrochemistry, renewable energy, and environmental science. He has worked on different research projects funded by various government agencies and universities and is the recipient of awards, including the Department of Science and Technology, India, Fast-Track Young Scientist Award and Young Researcher of the Year Award 2020 from Aligarh Muslim University. He has published about 210 research articles in various international scientific journals, many book chapters, and dozens of edited books, many with Wiley-Scrivener. Tariq Altalhi, PhD, is an associate professor in the Department of Chemistry at Taif University, Saudi Arabia. He received his doctorate degree from the University of Adelaide, Australia in 2014 with Dean’s Commendation for Doctoral Thesis Excellence. He has worked as head of the Chemistry Department at Taif University and Vice Dean of Science College. In 2015, one of his works was nominated for Green Tech awards from Germany, Europe’s largest environmental and business prize. He has also co-edited a number of scientific books. Abeer Alosaimi, PhD, is an assistant professor in the Department of Chemistry at Taif University, Saudi Arabia with over 22 years of academic experience. He has over 50 publications, including one book and articles in international journals and conferences. His research interests include polymer science, nanocomposites, and organic chemistry. Jorddy Neves Cruz is a researcher at the Federal University of Pará and the Emilio Goeldi Museum, Brazil. He has experience in multidisciplinary research in the areas of medicinal chemistry, drug design, extraction of bioactive compounds, extraction of essential oils, food chemistry and biological testing. He has published several research articles in scientific journals and is an associate editor of the Journal of Medicine.
