This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+ stem cell-derived mast cells, basophils from CD34+ progenitors, diagnostic applications, gene expression patterns in basophils, roles of basophils in different asthma phenotypes, knockout, and disease models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Basophils and Mast Cells: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Edited by:
Bernhard F. Gibbs, Franco H. Falcone Imprint: Springer-Verlag New York Inc. Country of Publication: United States Edition: 2nd ed. 2020 Volume: 2163 Dimensions:
Height: 254mm,
Width: 178mm,
Weight: 758g ISBN:9781071606988 ISBN 10: 1071606980 Series:Methods in Molecular Biology Pages: 380 Publication Date:08 August 2021 Audience:
Professional and scholarly
,
Undergraduate
Format:Paperback Publisher's Status: Active