Bacterial Transcriptional Control

Methods and Protocols

Irina Artsimovitch, Thomas J. Santangelo

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English

Humana Press Inc.
10 February 2015

Bacteriology (non-medical); Genetic engineering

Series: Methods in Molecular Biology

This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls.

Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future.

Edited by: Irina Artsimovitch, Thomas J. Santangelo
Imprint: Humana Press Inc.
Country of Publication: United States
Edition: 2015 ed.
Volume: 1276
Dimensions: Height: 254mm, Width: 178mm, Spine: 21mm
Weight: 8.924kg
ISBN: 9781493923915
ISBN 10: 1493923919
Series: Methods in Molecular Biology
Pages: 342
Publication Date: 10 February 2015
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

Name: Irina Artsimovitch Address: Department of Microbiology The Ohio State University 484 West 12th Avenue Columbus, OH 43210 Phone: (614) 292-6777 Email: artsimovitch.1@osu.edu