Bacterial Efflux Systems

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Ben Luisi (Department of Biochemistry, University of Cambridge, U.K.) Ben Luisi (Department of Biochemistry, University of Cambridge, U.K.)

Bacterial Efflux Systems

Name: Bacterial Efflux Systems
Price: 371.95 AUD
Availability: OutOfStock
ISBN: 9780443429590

Ben Luisi (Department of Biochemistry, University of Cambridge, U.K.), Ben Luisi (Department of Biochemistry, University of Cambridge, U.K.)

$371.95

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English

Academic Press Inc
15 December 2025

Biochemistry; Molecular biology

Series: Methods in Enzymology

Bacterial Efflux Systems, Volume 722 covers experimental and computational approaches to study the function, structure, and mechanism of bacterial efflux pump systems. The methods covered include preparation of samples for structural and functional analyses and in vivo activity analysis. Specific chapters in this release cover Efflux pump functional assays, NMR and functional analysis efflux pumps, Methods for saturation mutagenesis and functional characterization, Cooperation and functional interplay between pumps, Mapping interactions, HDX-MS, and much more.

Other sections cover Recombinant Expression and purification, Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach, Reconstitution of rnd and mfs efflux pumps for structural biology, CusCBA system, Expression and purification of efflux pumps of Gram positives, Expression and purification of efflux pumps of archaea, ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc, In situ structural analysis of efflux assemblies by cryoET, Molecular dynamics simulations, and so much more.

Volume editor: Ben Luisi (Department of Biochemistry University of Cambridge U.K.), Ben Luisi (Department of Biochemistry, University of Cambridge, U.K.)
Imprint: Academic Press Inc
Country of Publication: United States
Dimensions: Height: 229mm, Width: 152mm,
Weight: 450g
ISBN: 9780443429590
ISBN 10: 0443429596
Series: Methods in Enzymology
Pages: 564
Publication Date: 15 December 2025
Audience: Professional and scholarly , College/higher education , Undergraduate , Further / Higher Education
Format: Hardback
Publisher's Status: Active

Ben Luisi works in the Department of Biochemistry at the University of Cambridge, where he has an appointment as professor of structural biology. He and his colleagues study the structure and mechanism of efflux pumps and the riboregulatory machinery that help to control the expression of the pumps and other complex systems in bacteria.