Affinity Chromatography

Methods and Protocols

B. Vijayalakshmi Ayyar, Sushrut Arora

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English

Springer-Verlag New York Inc.
20 May 2023

Chromatography; Biology, life sciences

Series: Methods in Molecular Biology

This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.

Edited by: B. Vijayalakshmi Ayyar, Sushrut Arora
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States [Currently unable to ship to USA: see Shipping Info]
Edition: 2022 ed.
Volume: 2466
Dimensions: Height: 254mm, Width: 178mm,
Weight: 552g
ISBN: 9781071621783
ISBN 10: 1071621785
Series: Methods in Molecular Biology
Pages: 278
Publication Date: 20 May 2023
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active

Antibody Purification Using Affinity Chromatography.- TIM4-affinity Methods Targeting Phosphatidylserine for Isolation or Detection of Extracellular Vesicles.- Application of Affitins for Affinity Purification of Proteins.- Identification of Clusterin as a major ABri- and ADan-binding protein using affinity Chromatography.- Application of a Novel CL7/Im7 Affinity System in Purification of Complex and Pharmaceutical Proteins.- Positive and Negative Affinity Chromatography to Purify Norovirus P-Domain Protein.- Robotic Affinity Purification of Soluble and Insoluble Recombinant Glutathione-S-Transferase Fusion Proteins.- Application of Immunoaffinity-Mass Spectrometry (IA-MS) for Protein Biomarker Quantification.- Cellular Membrane Affinity Chromatography for the Identification of Bioactive Molecules from Complex Natural Mixtures.- Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP).- Arginine-affinity Chromatographyfor Nucleic Acids (DNA and RNA) Isolation.- Purification and Analysis of Nucleotides and Nucleosides from Plants.- Ligand Selection for Affinity Chromatography Using Phage Display.- Selection and Application of Aptamer Affinity for Protein Purification.- Entrapment of Proteins within Columns for High-Performance Affinity Chromatography.- Preparation of Affinity Chromatography Monolith in Miniaturized Format and Application for Protein Separation.- Ligand Immobilization Methods for Affinity Chromatography.- Preparation of Molecularly Imprinted poly(N-isopropylacrylamide) Thermosensitive Based Cryogels.- Preparation of Staphylococcal Protein a Imprinted Supermacroporous Cryogel Beads.