Advanced Protocols for Animal Transgenesis

An ISTT Manual

Shirley Pease, Thomas L. Saunders

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Hardback

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English

Springer-Verlag Berlin and Heidelberg GmbH & Co. K
31 August 2011

Genetics (non-medical); Animal reproduction

Series: Springer Protocols Handbooks

Summary
Details

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Edited by: Shirley Pease, Thomas L. Saunders
Imprint: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Country of Publication: Germany
Edition: 2011
Dimensions: Height: 254mm, Width: 178mm, Spine: 43mm
Weight: 1.473kg
ISBN: 9783642207914
ISBN 10: 364220791X
Series: Springer Protocols Handbooks
Pages: 669
Publication Date: 31 August 2011
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active