A Structure-Function Toolbox for Membrane Transporter and Channels

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Christine Ziegler (Department of Structural Biology, Max Planck Institute of Biophysics, Germany) Christine Ziegler (Department of Structural Biology, Max Planck Institute of Biophysics, Germany)

A Structure-Function Toolbox for Membrane Transporter and Channels

Christine Ziegler (Department of Structural Biology, Max Planck Institute of Biophysics, Germany), Christine Ziegler (Department of Structural Biology, Max Planck Institute of Biophysics, Germany), Christine Ziegler (Department of Structural Biology, Max Planck Institute of Biophysics, Germany) , Christine Ziegler (Department of Structural Biology, Max Planck Institute of Biophysics, Germany)

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English

Academic Press Inc
04 August 2017

Physiology; Clinical & internal medicine; Enzymology; Molecular biology

Series: Methods in Enzymology

A Structure-Function Toolbox for Membrane Transporter and Channels, Volume 594, a new release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET on transporters, Dynamics of channels and transporters investigated by NMR, Structure-function studies on channels and transporters, and a section on MemStar, a new GFP-based expression and purification system for transporters and channels.

Volume editor: Christine Ziegler (Department of Structural Biology Max Planck Institute of Biophysics Germany), Christine Ziegler (Department of Structural Biology, Max Planck Institute of Biophysics, Germany), Christine Ziegler (Department of Structural Biology, Max Planck Institute of Biophysics, Germany), Christine Ziegler (Department of Structural Biology, Max Planck Institute of Biophysics, Germany)
Imprint: Academic Press Inc
Country of Publication: United States
Dimensions: Height: 229mm, Width: 151mm,
Weight: 610g
ISBN: 9780128123539
ISBN 10: 0128123532
Series: Methods in Enzymology
Pages: 302
Publication Date: 04 August 2017
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

1. Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM 2. SSM-Based Electrophysiology for Transporter Research 3. Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters 4. Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy 5. The MEMbrane Protein Single ShoT Amplification Recipe: MemStar 6. Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli 7. Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling 8. Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR 9. Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein

Prof. Dr. Christine Ziegler works at the Department of Structural Biology, Max Planck Institute of Biophysics, Germany

Reviews for A Structure-Function Toolbox for Membrane Transporter and Channels

"Praise for the Series: ""Should be on the shelves of all libraries in the world as a whole collection."" --Chemistry in Industry ""The work most often consulted in the lab."" --Enzymologia ""The Methods in Enzymology series represents the gold-standard."" --Neuroscience"