Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols

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Paula Meleady (Senior Research Scientist, Programme Leader, Proteomics Core Facility; Associate Director National Institute for Cell Biology, Ireland)

Proteomics Mass Spectrometry Methods

Sample Preparation, Protein Digestion, and Research Protocols

Paula Meleady (Senior Research Scientist, Programme Leader, Proteomics Core Facility; Associate Director National Institute for Cell Biology, Ireland)

$220.95

Paperback

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English

Academic Press Inc
16 February 2024

Cellular biology (cytology)

Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols shares best practices collected across key laboratories and core facilities, taking the reader through key tactics for executing the most usual mass spectrometry experiments. Sections review research making use of MS proteomics experiments, focus on critical sample preparation, cover mammalian cell lines and samples from clinical tissue and biological fluids, discuss subcellular fractionation, provide methods for protein digestion both for in gel and in solution, and delve into key MS proteomics analysis protocols, including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, and more. This book is the perfect lab manual for research teams or for use as a new staff training material. Core facility managers may also find it useful for sharing best practices with their staff and researchers.

Edited by: Paula Meleady (Senior Research Scientist Programme Leader Proteomics Core Facility; Associate Director National Institute for Cell Biology Ireland)
Imprint: Academic Press Inc
Country of Publication: United Kingdom
Dimensions: Height: 235mm, Width: 191mm,
Weight: 450g
ISBN: 9780323903950
ISBN 10: 0323903959
Pages: 240
Publication Date: 16 February 2024
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active

Dr. Meleady received her degree in Biotechnology from the Dublin City University and PhD from the National Cell and Tissue Culture Centre. She is currently Senior Research Scientist, Programme Leader, Proteomics Core Facility, and Associate Director National Institute for Cell Biology.

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Proteomics Mass Spectrometry Methods

Sample Preparation, Protein Digestion, and Research Protocols

Paula Meleady (Senior Research Scientist, Programme Leader, Proteomics Core Facility; Associate Director National Institute for Cell Biology, Ireland)

$220.95

Paperback

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