Fish, and particularly zebrafish, have become the fastest-growing segment of the research population. They offer several advantages, in terms of biology and technologies to apply, and thus are employed in numerous research fields. Laboratory Fish in Biomedical Research: Biology, Husbandry and Research Applications for Zebrafish, Medaka, Killifish, Swordtail Fish, Cavefish, Stickleback, Goldfish and Danionella Translucida addresses the relevant and increasing need to collect cutting-edge knowledge on husbandry, maintenance, welfare and experimental protocols of the most common freshwater species under standard laboratory conditions.
Section 1: Zebrafish (Danio rerio) 1. Biology and research applications Brautigam Lars and Filipek-Go´rniok Beata 2. Housing and maintenance of zebrafish, new technologies in laboratory aquatic systems and considerations for facility design Bruce Newell and Marco Brocca 3. Breeding and larviculture of zebrafish (Danio rerio) Benjamin Tsang and Robert Gerlai 4. Health monitoring, disease, and clinical pathology Jean-Philippe Mocho and Nuno Pereira 5. The welfare of zebrafish Paul Georg Schroeder 6. Analgesia, anesthesia, and euthanasia in zebrafish Almut Kohler and Ana M. Valentim 7. Transgenesis, mutagenesis, knockdown, and genetic colony management Claire Allen, Francesco Argenton, Fredericus J. Van Eeden and Natascia Tiso 8. Sperm cryopreservation, in vitro fertilization, and embryo freezing Kanav Khosla, John Bischof and Zolta´n M. Varga Section 2: Other small freshwater fish 9. Medaka as a model teleost: characteristics and approaches of genetic modification Tokiro Ishikawa, Yu Murakami, Chika Fujimori, Masato Kinoshita, Kiyoshi Naruse and Shinji Kanda 10. Integrated analyses using medaka as a powerful model animal toward understanding various aspects of reproductive regulation Chie Umatani, Mikoto Nakajo, Daichi Kayo, Yoshitaka Oka and Shinji Kanda 11. The African turquoise killifish (Nothobranchius furzeri): biology and research applications Gwendoline Astre, Eitan Moses and Itamar Harel 12. Challenges in keeping annual killifish Martin Reichard, Radim Blazek, Iva Dykova, Jakub Zak and Matej Polacik 13. Mexican tetra (Astyanax mexicanus): biology, husbandry, and experimental protocols Diana P. Baumann and Andrew Ingalls 14. The housing, care, and use of a laboratory three-spined stickleback colony Ellen Blaker, Marion Sebire, Tim Ellis and Ioanna Katsiadaki 15. Goldfish (Carassius auratus): biology, husbandry, and research applications Ayelen M. Blanco and Suraj Unniappan 16. Danionella translucida, a tankful of new opportunities Gokul Rajan, Karine Duroure and Filippo Del Bene Section 3: Databases 17. Fish inventory databases Jana Oltova
Livia D’Angelo is veterinarian by background, she is researcher at University of Naples. Her research activities are mainly focused on the neurobiology of two freshwater fish, zebrafish and the turquoise killifish. She is also deeply involved in the laboratory animal sciences field, with respect to husbandry and management of aquatic research organisms. Paolo de Girolamo is a veterinarian by background, he is full Professor at University of Naples, and he is in charge of Chief of Veterinary Officer and Ethical Animal Care and Use Committee. His research interests have been since long addressed to the neurobiology and gut-brain axis interplay in teleostean fish (goldfish, zebrafish, turquoise killifish etc.).
