Biomedical photonics is currently one of the fastest growing fields, connecting research in physics, optics, and electrical engineering coupled with medical and biological applications. It allows for the structural and functional analysis of tissues and cells with resolution and contrast unattainable by any other methods.
However, the major challenges of many biophotonics techniques are associated with the need to enhance imaging resolution even further to the sub-cellular level as well as translate them for in vivo studies. The tissue optical clearing method uses immersion of tissues into optical clearing agents (OCAs) that reduces the scattering of tissue and makes tissue more transparent and this method has been successfully used ever since.
This book is a self-contained introduction to tissue optical clearing, including the basic principles and in vitro biological applications, from in vitro to in vivo tissue optical clearing methods, and combination of tissue optical clearing and various optical imaging for diagnosis. The chapters cover a wide range of issues related to the field of tissue optical clearing: mechanisms of tissue optical clearing in vitro and in vivo; traditional and innovative optical clearing agents; recent achievements in optical clearing of different tissues (including pathological tissues) and blood for optical imaging diagnosis and therapy.
This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods.
The book is addressed to biophysicist researchers, graduate students and postdocs of biomedical specialties, as well as biomedical engineers and physicians interested in the development and application of optical methods in medicine.
Key features:
The first collective reference to collate all known knowledge on this topic
Edited by experts in the field with chapter contributions from subject area specialists
Brings together the two main approaches in immersion optical clearing into one cohesive book
Preface............................................................................................................................................................................................. ix Acknowledgments..........................................................................................................................................................................xiii Editors............................................................................................................................................................................................. xv Contributors..................................................................................................................................................................................xvii Part I Basic principles of tissue optical clearing 1. Tissue optical clearing mechanisms...................................................................................................................................... 3 Tingting Yu, Dan Zhu, Luís Oliveira, Elina A. Genina, Alexey N. Bashkatov, and Valery V. Tuchin 2. Tissue optical clearing for Mueller matrix microscopy.................................................................................................... 31 Nan Zeng, Honghui He, Valery V. Tuchin, and Hui Ma 3. Traditional and innovative optical clearing agents........................................................................................................... 67 Elina A. Genina, Vadim D. Genin, Jingtan Zhu, Alexey N. Bashkatov, Dan Zhu, and Valery V. Tuchin 4. Chemical enhancers for improving tissue optical clearing efficacy................................................................................. 93 Dan Zhu, Yanmei Liang, Xingde Li, and Valery V. Tuchin 5. Human skin autofluorescence and optical clearing......................................................................................................... 109 Walter Blondel, Marine Amouroux, Sergey M. Zaytsev, Elina A. Genina, Victor Colas, Christian Daul, Alexander B. Pravdin, and Valery V. Tuchin 6. Molecular modeling of post-diffusion phase of optical clearing of biological tissues.................................................. 127 Kirill V. Berezin, Konstantin N. Dvoretskiy, Maria L. Chernavina, Anatoly M. Likhter, and Valery V. Tuchin 7. Refractive index measurements of tissue and blood components and OCAs in a wide spectral range......................141 Ekaterina N. Lazareva, Luís Oliveira, Irina Yu. Yanina, Nikita V. Chernomyrdin, Guzel R. Musina, Daria K. Tuchina, Alexey N. Bashkatov, Kirill I. Zaytsev, and Valery V. Tuchin 8. Water migration at skin optical clearing...........................................................................................................................167 Anton Yu. Sdobnov, Johannes Schleusener, Jürgen Lademann, Valery V. Tuchin, and Maxim E. Darvin 9. Optical and mechanical properties of cartilage during optical clearing...................................................................... 185 Yulia M. Alexandrovskaya, Olga I. Baum, Vladimir Yu. Zaitsev, Alexander A. Sovetsky, Alexander L. Matveyev, Lev A. Matveev, Kirill V. Larin, Emil N. Sobol, and Valery V. Tuchin 10. Compression optical clearing............................................................................................................................................. 199 Olga A. Zyuryukina and Yury P. Sinichkin Part II Tissue optical clearing method for biology (3D imaging) 11. Optical clearing for multiscale tissues and the quantitative evaluation of clearing methods in mouse organs..............221 Tingting Yu, Jianyi Xu, and Dan Zhu 12. Ultrafast aqueous clearing methods for 3D imaging....................................................................................................... 239 Tingting Yu, Jingtan Zhu, and Dan Zhu 13. Challenges and opportunities in hydrophilic tissue clearing methods.......................................................................... 257 Etsuo A. Susaki and Hiroki R. Ueda 14. Combination of tissue optical clearing and 3D fluorescence microscopy for high-throughput imaging of entire organs and organisms ......................................................................................................................................... 277 Peng Fei and Chunyu Fang 15. Endogenous fluorescence preservation from solvent-based optical clearing................................................................ 297 Tingting Yu, Yisong Qi, and Dan Zhu 16. Progress in ex situ tissue optical clearing – shifting immuno-oncology to the third dimension..................................315 Paweł Matryba, Leszek Kaczmarek, and Jakub Gołąb Part III Towards in vivo tissue optical clearing 17. In vivo skin optical clearing methods for blood flow and cell imaging.......................................................................... 333 Dongyu Li, Wei Feng, Rui Shi, and Dan Zhu 18. In vivo skull optical clearing for imaging cortical neuron and vascular structure and function................................351 Dongyu Li, Yanjie Zhao, Chao Zhang, and Dan Zhu 19. In vivo skin optical clearing in humans............................................................................................................................. 369 Elina A. Genina, Alexey N. Bashkatov, Vladimir P. Zharov, and Valery V. Tuchin 20. Optical clearing of blood and tissues using blood components...................................................................................... 383 Olga S. Zhernovaya, Elina A. Genina, Valery V. Tuchin, and Alexey N. Bashkatov 21. Blood and lymph flow imaging at optical clearing........................................................................................................... 393 Polina A. Dyachenko (Timoshina), Arkady S. Abdurashitov, Oxana V. Semyachkina-Glushkovskaya, and Valery V. Tuchin Part IV Combination of tissue optical clearing and optical imaging/spectroscopy for diagnostics 22. Optical clearing aided photoacoustic imaging in vivo......................................................................................................411 Yong Zhou and Lihong V. Wang 23. Enhancement of contrast in photoacoustic – fluorescence tomography and cytometry using optical clearing and contrast agents...................................................................................................................................419 Julijana Cvjetinovic, Daniil V. Nozdriukhin, Maksim Mokrousov, Alexander Novikov, Marina V. Novoselova, Valery V. Tuchin, and Dmitry A. Gorin 24. Tissue optical clearing in the terahertz range.................................................................................................................. 445 Olga A. Smolyanskaya, Kirill I. Zaytsev, Irina N. Dolganova, Guzel R. Musina, Daria K. Tuchina, Maxim M. Nazarov, Alexander P. Shkurinov, and Valery V. Tuchin 25. Magnetic resonance imaging study of diamagnetic and paramagnetic agents for optical clearing of tumor-specific fluorescent signal in vivo....................................................................................................................... 459 Alexei A. Bogdanov Jr., Natalia I. Kazachkina, Victoria V. Zherdeva, Irina G. Meerovich, Daria K. Tuchina, Ilya D. Solovyev, Alexander P. Savitsky, and Valery V. Tuchin 26. Use of optical clearing and index matching agents to enhance the imaging of caries, lesions, and internal structures in teeth using optical coherence tomography and SWIR imaging........................................ 471 Daniel Fried 27. Optical clearing of adipose tissue...................................................................................................................................... 487 Irina Yu. Yanina, Yohei Tanikawa, Daria K. Tuchina, Polina A. Dyachenko (Timoshina), Yasunobu Iga, Shinichi Takimoto, Elina A. Genina, Alexey N. Bashkatov, Georgy S. Terentyuk, Nikita A. Navolokin, Alla B. Bucharskaya, Galina N. Maslyakova, and Valery V. Tuchin 28. Diabetes mellitus-induced alterations of tissue optical properties, optical clearing efficiency, and molecular diffusivity.....................................................................................................................................................517 Daria K. Tuchina and Valery V. Tuchin 29. Tissue optical clearing for in vivo detection and imaging diabetes induced changes in cells, vascular structure, and function....................................................................................................................................... 539 Dongyu Li, Wei Feng, Rui Shi, Valery V. Tuchin, and Dan Zhu 30. Light operation on cortex through optical clearing skull window................................................................................. 557 Dongyu Li, Chao Zhang, Oxana Glushkovskaya, Yanjie Zhao, and Dan Zhu 31. The role of optical clearing to enhance the applications of in vivo OCT and photodynamic therapy: Towards PDT of pigmented melanomas and beyond...................................................................................................... 569 Layla Pires, Michelle Barreto Requena, Valentin Demidov, Ana Gabriela Salvio, I. Alex Vitkin, Brian C. Wilson, and Cristina Kurachi 32. Combination of tissue optical clearing and OCT for tumor diagnosis via permeability coefficient measurements.................................................................................................................................................... 577 Qingliang Zhao 33. Optical clearing for cancer diagnostics and monitoring................................................................................................. 597 Luís M. Oliveira and Valery V. Tuchin 34. Contrast enhancement and tissue differentiation in optical coherence tomography with mechanical compression............................................................................................................................................ 607 Pavel D. Agrba and Mikhail Yu. Kirillin 35. Measurement of the dermal beta-carotene in the context of multimodal optical clearing..........................................619 Mohammad Ali Ansari and Valery V. Tuchin 36. Optical clearing and molecular diffusivity of hard and soft oral tissues...................................................................... 629 Alexey A. Selifonov and Valery V. Tuchin 37. Optical clearing and Raman spectroscopy: In vivo applications................................................................................... 647 Qingyu Lin, Ekaterina N. Lazareva, Vyacheslav I. Kochubey, Yixiang Duan, and Valery V. Tuchin Index............................................................................................................................................................................................. 655
Valery V. Tuchin, Dan Zhu, Elina A. Genina
Reviews for Handbook of Tissue Optical Clearing: New Prospects in Optical Imaging
The book—subtitled “New Prospects in Optical Imaging”—is unique in bringing together a number of diverse tissue optical clearing (TOC) topics, such as 3D imaging and the combination of TOC and optical imaging/spectroscopy for diagnostics, and presenting it all at a level suitable for graduate students in biophysics and engineering. This task is accomplished with an emphasis on both methodology and applications. I found the description of these basic biophotonics techniques particularly detailed and interesting. Although written by more than 100 contributors, the style of the book is generally clear and fluent. Review by Christian Brosseau, Optica Fellow and professor of physics, Université de Bretagne Occidentale, Brest, France, June 2022.
