PCR Technology: Current Innovations, Third Edition

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Tania Nolan (Sigma Life Science Custom Products, Haverhill, UK) Stephen A. Bustin

PCR Technology

Current Innovations, Third Edition

Tania Nolan (Sigma Life Science Custom Products, Haverhill, UK), Stephen A. Bustin

$410

Hardback

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English

CRC Press Inc
13 June 2013

DNA & Genome; Genetic engineering

PCR’s simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that provides a critical and timely reference point for anyone wishing to use this technology.

Topics in this forward-thinking volume include:

The purification and handling of PCR templates The effect of the manufacture and purification of the oligonucleotide on PCR behavior Optimum buffer composition Probe options The design and optimization of qPCR assays Issues surrounding the development and refinement of instrumentation Effective controls to protect against uncertainties due to reaction variability

Covering all aspects of PCR and real-time PCR, the book contains detailed protocols that make it suitable as both a reference and an instruction manual. Each chapter presents detailed guidelines as well as helpful hints and tips supplied by authors who are recognized experts in their fields. In addition to descriptions of current technology and best practices, the book also provides information about new developments in the PCR arena.

Edited by: Tania Nolan (Sigma Life Science Custom Products Haverhill UK), Stephen A. Bustin
Imprint: CRC Press Inc
Country of Publication: United States
Edition: 3rd edition
Dimensions: Height: 254mm, Width: 178mm, Spine: 28mm
Weight: 975g
ISBN: 9781439848050
ISBN 10: 143984805X
Pages: 470
Publication Date: 13 June 2013
Audience: College/higher education , Professional and scholarly , Further / Higher Education , Undergraduate
Format: Hardback
Publisher's Status: Active

"Dr. Tania Nolan is an internationally recognized molecular biologist with a reputation for expertise in the field of mRNA quantification using RT-qPCR. She was a significant coauthor of the specialist textbook The A–Z of Quantitative PCR along with numerous other book chapters and peer-reviewed papers. She has presented several plenary lectures and chaired sessions at major international qPCR meetings. Stephen A. Bustin, BS(Mod) PhD FSB acquired his first qPCR instrument in 1997 and has published numerous peer-reviewed papers that describe and use this technology. He wrote and edited the A–Z of Quantitative PCR, universally acknowledged as the ""qPCR Bible,"" edited The PCR Revolution, and has written a series of Definitive qPCR ebooks. He also led the international consortium that drew up the MIQE guidelines."

Reviews for PCR Technology: Current Innovations, Third Edition

Praise for Previous Editions This ... second edition provides up-to-date information on PCR technology and applications and covers a selection of widely used procedures and approaches for qualitative as well as quantitative queries. It certainly offers easy to follow, step-by-step protocols and explains the parameters governing PCR reaction outcomes ... ... [A] fundamental reference for scientists new to PCR technology and a source of up-to-date applications for those familiar with the method ... I highly recommend this book as a useful tool for hospital scientists who may use PCR technology in their work; or for scientific researchers ... - Australian Journal of Medical Science, Nov. 2004, Vol. 25, No.4