Arousal states are processes that include waking, deep sleep, and the dreaming phase of sleep (REM). Molecular Regulation of Arousal States explores the cellular and molecular mechanisms by which sleep and wakefulness are regulated and seeks explanations for the generation of arousal states. It presents step-by-step research protocols that allow investigators to apply the techniques described to a wide range of physiological and behavioral research problems, such as sleep neurobiology and state-dependent disruption of cardiopulmonary control. For the first time, a single source integrates cellular and molecular research techniques with studies of arousal, opening the door to exciting new research methodologies.
Chapter 1. Application of In Situ Hybridization to the Study of Rhythmic Neural Systems /H. Elliott Albers, R. Thomas Zoeller, and Kim L. Huhman -- Chapter 2. Estimation of the mRNAs Encoding the Cholinergic Muscarinic Receptor and Acetylcholine Vesicular Transport Proteins Involved in Central Cardiovascular Regulation /Jerry J. Buccafusco, Lu C. Zhang, and Mark A. Prendergast -- Chapter 3. Voltammetric Detection of Nitric Oxide (NO) in the Rat Brain: Release Throughout the Sleep-Wake Cycle /Sophie Burlet and Raymond Cespuglio -- Chapter 4. Sleep Regulatory Substances: Change in mRNA Expression Linked to Sleep /Zutang Chen and James M. Krueger -- Chapter 5. Immediate Early Genes as a Tool to Understand the Regulation of the Sleep-Waking Cycle: Immunocytochemistry, In Situ Hybridization, and Antisense Approaches /Chiara Cirelli, Maria Pompeiano, and Giulio Tononi -- Chapter 6. Methods for the Measurement of Adenylyl Cyclase Activity /Charles W. Emala -- Chapter 7. Methods Used to Assess Specific Messenger RNA Expression During Sleep /Mary Ann Greco, Lalini Ramanathan, Radhika Basheer, and Priyattam J. Shiromani -- Chapter 8. Competition Binding Assays for Determining the Affinity and Number of Muscarinic Receptor Subtypes in Tissue Homogenates /A. Urban Hoglund and Helen A. Baghdoyan -- Chapter 9. Isolation and Identification of Specific Transcripts by Subtractive Hybridization /Thomas S. Kilduff, Luis de Lecea, Hiroshi Usui, and J. Gregor Sutcliffe -- Chapter 10. Use of In Situ Hybridization Histochemistry to Study Muscarinic Receptor mRNA Expression in Brains of Sleep-Deprived Rats /Clete Kushida and Donna M. Simmons -- Chapter 11. Transcriptional Regulation of Putative Sleep-Promoting Compounds /Miroslaw Mackiewicz, Sigrid C. Veasey, and Allan I. Pack -- Chapter 12. Chemical Mutagenesis and Screening for Mouse Mutations with an Altered Rest-Activity Pattern /Patrick M. Nolan, Thomas A. Houpt, and Maja Bucan -- Chapter 13. Reverse Transcription mRNA Differential Display: A Systematic Molecular Approach to Identify Changes in Gene Expression Across the Sleep-Waking Cycle /Maria Pompeiano, Chiara Cirelli, and Giulio Tononi -- Chapter 14. In Situ Hybridization of Messenger RNA in Sleep Research /Tarja Porkka-Heiskanen, Jussi Toppila, and Dag Stenberg -- Chapter 15. New Directions in the Analysis of Brain Substances Related to Sleep and Wakefulness /Gary Siuzdak and Steven Henriksen -- Chapter 16. Sleep and Circadian Rest-Activity Rhythms in Prion Protein Knockout Mice /Irene M. Tobler; Marek Fischer; and Jean C. Manson -- Chapter 17. Measurement of Nitric Oxide in the Brain Using the Hemoglobin Trapping Technique Coupled with In Vivo Microdialysis /Julie A. Williams, Steven R. Vincent, and Peter B. Reiner -- Chapter 18. Mapping Regional Cerebral Protein Synthesis During Sleep /Rebecca K. Zoltoski -- Index.
