In Situ Hybridization in Electron Microscopy

Gerard Morel, Annie Cavalier, Lynda Williams (The Rowett Research Institute, Aberdeen, UK)

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English

CRC Press Inc
30 January 2001

DNA & Genome; Microbiology (non-medical)

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in

individual cells, and is an especially important method for

studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes: hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method) hybridization prior to embedding (pre-embedding) hybridization on ultrathin sections of frozen tissue (frozen tissue method). For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization,

and visualization of the hybridization products. The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.

By: Gerard Morel, Annie Cavalier, Lynda Williams (The Rowett Research Institute, Aberdeen, UK)
Imprint: CRC Press Inc
Country of Publication: United States
Dimensions: Height: 254mm, Width: 178mm, Spine: 28mm
Weight: 1.150kg
ISBN: 9780849300448
ISBN 10: 0849300444
Pages: 472
Publication Date: 30 January 2001
Audience: Professional and scholarly , Professional and scholarly , Professional & Vocational , Undergraduate , Further / Higher Education
Format: Hardback
Publisher's Status: Active

General Introduction. Abbreviations. Probes. Principles of Methodology. Sample Preparation. Post-Embedding Technique. Pre-Embedding Technique. Frozen Tissue Technique. Semithin Sections. Controls and Problems. Examples of Results. Appendices. Glossary. Index.

Morel, Gerard | Cavalier, Annie | Williams, Lynda